Two-Dimensional Liquid Chromatography (2D-LC) for Biopharmaceuticals

Quantifying Biopharmaceuticals In Biological Samples advances in liquid chromatography–tandem mass Spectrometry (lc–mS–mS)–Based Quantitation of Biopharmaceuticals in Biological Samples

Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing.

The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a production bottleneck that is shifting the costs of production. This review focuses on the use of aqueou...

Orthogonality of separation in two-dimensional liquid chromatography.

Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC modes for peptides is not well known, and mathematical characterization for orthogonality is unde...

Correspondence Quantification in Comprehensive Two-Dimensional Liquid Chromatography

This correspondence corrects the description in a recent paper by Mondello et al., “Quantification in Comprehensive Two-Dimensional Liquid Chromatography” [Mondello, L.; Herrero, M.; Kumm, T.; Dugo, P.; Cortes, H.; Dugo, G. Anal. Chem., 2008, 80, 5418-5424], of previous research on peak integration. This correspondence also shows that the peak integration method proposed in that paper is equiva...

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Online comprehensive two-dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two-dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two-dimensional liquid ...